By Martin J. Pfeiffer, Martin Stehling, Anna Jauch (auth.), Hossein Baharvand, Nasser Aghdami (eds.)
Advances in Stem phone Research discusses fresh advances in stem mobile technology, together with healing purposes. This quantity covers such issues as biomanufacturing iPS cells for healing functions, innovations for controlling stem mobile destiny judgements, in addition to present easy learn in such components as germ line stem cells, genomics and proteomics in stem phone learn. it's a invaluable ebook for biology and medical scientists, specially younger investigators and stem telephone biology scholars who're newly getting into the realm of stem cells study. The editors desire that the recent wisdom and examine defined during this ebook might help give a contribution to new treatments for a large choice of illnesses that shortly afflict humanity.
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PLoS ONE 3(9):e3294 Werbowetski-Ogilvie TE, Bosse M, Stewart M, Schnerch A, Ramos-Mejia V, Rouleau A, Wynder T, Smith MJ, Dingwall S, Carter T, Williams C, Harris C, Dolling J, Wynder C, Boreham D, Bhatia M (2009) Characterization of human embryonic stem cells with features of neoplastic progression. Nat Biotechnol 27(1):91–97 Mitalipova MM, Rao RR, Hoyer DM, Johnson JA, Meisner LF, Jones KL, Dalton S, Stice SL (2005) Preserving the genetic integrity of human embryonic stem cells. Nat Biotechnol 23(1):19–20 Ben-Porath I, Thomson MW, Carey VJ, Ge R, Bell GW, Regev A, Weinberg RA (2008) An embryonic stem cell-like gene expression signature in poorly differentiated aggressive human tumors.
If one assumes that for a mutation to reach detectable levels it must bestow a growth advantage on the variant cell, then the identification of culture adapted ES cells could be achieved based on mutation. With karyotyping services readily available and array-CGH becoming more accessible, any change in DNA content can be observed. In addition, the advent of sequencing technologies may also allow identification of point mutations, or any novel splice variants. However, to demonstrate that a mutant has spread through a population requires regular monitoring, and the procedures to detect mutations are destructive to the cells.
However, it is possible to fabricate polymers that bind hESCs on tissue culture polystyrene dishes to facilitate hESC culture. Villa-Diaz et al. identified that poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH) was able to maintain various hESC and hiPSC lines . hESCs grown on PMEDSAH-coated plates in MEF-conditioned medium remained in an undifferentiated state, maintain pluripotency, and did not acquire an abnormal karyotype. However, this substrate was not effective with all cell lines and media formulations tested.
Advances in Stem Cell Research by Martin J. Pfeiffer, Martin Stehling, Anna Jauch (auth.), Hossein Baharvand, Nasser Aghdami (eds.)